The protein sample. Purity of the protein sample. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) Sample homogeneity. Protein concentration and starting buffer. Protein assays Detergent. Detergent to protein ratio. Crystallization methods Vapor diffusion. Equilibrium dialysis. Equipment and other tools Screening for crystals Initial screen Adjusted screen Optimization screen Additives Description of what you see in the drop Examination of the crystallization experiments. Crystallization drop phenomena and interpretation. References
