X-tal fact2
Date: .. - .. - .. Temperature: .... °C. Experiment number:..........
Experiment name:...........................................................
Protein : .................................................................
[protein] : ........ mg/ml
protein volume : ........ ul
Mother liquor : ........ ul
Buffer ....................................................................
| pH | additive | precipitant1 | precipitant2 | result |
1A1 | 5.5 | 10 % DIOXANE | 2.0 M (NH4)2SO4 | | .............. |
1B1 | 7.5 | 1 % DIOXANE | 20 % PEG 6000 | | .............. |
1C1 | 9.0 | 10 % DIOXANE | 20 % Isopropanol | | .............. |
1D1 | 6.5 | 20 % DIOXANE | | | .............. |
1A2 | 8.0 | 10 % DMSO | 2.45 M (NH4)2SO4 | | .............. |
1B2 | 9.0 | 10 % DMSO | 10 % MME-PEG 5000 | 10 % MPD | .............. |
1C2 | 7.0 | 10 % DMSO | 20 % MPD | | .............. |
1D2 | 6.0 | 30 % DMSO | 10 % Jeffamine | | .............. |
1A3 | 8.5 | 3.0 M CsCl | 1.0 M (NH4)2SO4 | | .............. |
1B3 | 4.5 | 200 mM CsCl | 15 % MME-PEG 5000 | | .............. |
1C3 | 7.0 | 200 mM CsCl | 2.0 M Hexane-1,6-diol | | .............. |
1D3 | 6.5 | 3.0 M CsCl | | | .............. |
1A4 | 5.5 | 2.5 % 1-octanol | 1.5 M (NH4)2SO4 | | .............. |
1B4 | 7.5 | 2.5 % 1-octanol | 15 % PPG P-425 | | .............. |
1C4 | 6.0 | 2.5 % 1-octanol | 20 % Isopropanol | | .............. |
1D4 | 8.0 | 2.5 % 1-octanol | 1.0 M KCl | | .............. |
1A5 | 8.5 | 10 % Glycerol | 2.45 M (NH4)2SO4 | | .............. |
1B5 | 6.0 | 20 % Glycerol | 10 % PEG 6000 | | .............. |
1C5 | 7.0 | 10 % Glycerol | 20 % MPD | | .............. |
1D5 | 5.5 | 10 % Glycerol | 2.0 M NaCl | | .............. |
1A6 | 8.0 | 0.8 % ß-octyl glucoside | 2.0 M (NH4)2SO4 | 20 % Ethyleneglycol | .............. |
1B6 | 7.5 | 0.1 % ß-octyl glucoside | 15 % PEG 6000 | | .............. |
1C6 | 6.5 | 0.1 % ß-octyl glucoside | 20 % MPD | | .............. |
1D6 | 4.5 | 0.5 % ß-octyl glucoside | 3.0 M NaCL | | .............. |
|
2A1 | 5.5 | 0.01 % NP-40 | 1.5 M (NH4)2SO4 | | .............. |
2B1 | 7.0 | 0.01 % NP-40 | 20 % MME-PEG 5000 | | .............. |
2C1 | 4.5 | 0.01 % NP-40 | 20 % MPD | 1.0 M (NH4)2SO4 | .............. |
2D1 | 8.5 | 0.01 % NP-40 | 2.0 M LiCl2 | | .............. |
2A2 | 6.0 | 200 mM Cobalt Sulfate | 1.0 M (NH4)2SO4 | | .............. |
2B2 | 4.5 | 50 mM Cobalt Sulfate | 15 % Jeffamine M-600 | | .............. |
2C2 | 7.5 | 200 mM Cobalt Sulfate | 1.5 M Hexane-1,6-diol | | .............. |
2D2 | 7.0 | 1.5 M Cobalt Sulphate | | | .............. |
2A3 | 8.0 | 1.0 M NaCl | 2.0 M (NH4)2SO4 | | .............. |
2B3 | 6.5 | 2.0 M NaCl | 10 % PEG 6000 | 10 % MME-PEG 5000 | .............. |
2C3 | 7.5 | 200 mM NaCl | 1.5 M Hexane-1,6-diol | | .............. |
2D3 | 6.0 | 2.0 M NaCl | 30 % Ethyleneglycol | | .............. |
2A4 | 8.0 | 50 mM MgCl2, 50 mM KCl | 1.5 M (NH4)2SO4 | 10 % glycerol | .............. |
2B4 | 4.5 | 50 mM MgCl2, 50 mM KCl | 20 % Jeffamine M-600 | | .............. |
2C4 | 6.5 | 50 mM MgCl2, 50 mM KCl | 30 % MPD | | .............. |
2D4 | 7.0 | 1.0 M MgCl2 | 1.0 M KCl | | .............. |
2A5 | 7.0 | 2.5 M LiCl2 | 200 mM (NH4)2SO4 | | .............. |
2B5 | 5.5 | 1.0 M LiCl2 | 20 % Ethyleneglycol | | .............. |
2C5 | 9.0 | 1.0 M LiCl2 | 20 % PPG P-425 | | .............. |
2D5 | 8.5 | 1.0 M LiCl2 | 1.0 M NaCl | | .............. |
2A6 | 5.5 | 1.0 M KCl | 1.0 M (NH4)2SO4 | | .............. |
2B6 | 8.0 | 200 mM KCl | 20 % PEG 6000 | 20 % Ethyleneglycol | .............. |
2C6 | 7.0 | 200 mM KCl | 20 % Jeffamine M-600 | | .............. |
2D6 | 6.0 | 2.0 M KCl | | | .............. |
© 16 Febr 2004 Johan Zeelen