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X-tal protocols

stock solutions X-tal fact1

Protein Detergent Methods Euipment Screen Drops Reference
1.H20
2.50 % (V/V)2-Methyl-2,4-pentanediol (MPD)
3.50 % (V/V)Polyethylene glycol (PEG) 400
4.40 % (W/V)Polyethylene glycol (PEG) 1500
5.40 % (W/V)Polyethylene glycol (PEG) 6000
6.1.0 MAcetic acid/NaOHpH=4.5
7.1.0 MCitric Acid/NaOHpH=5.5
8.1.0 MAda (N-[2-Acetamido]-2-iminodiacetic acid) /NaOH pH=6.5
9.1.0 M Tea ( Triethanol-amine )/HClpH=7.5
10.1.0 MTris ( tris{Hydroxymethyl]aminomethane)/HClpH=8.5
11.1.0 MChes (2-[NN-Cyclohexylamino]ethane-sulfonic acid)/NaOHpH=9.5
12.1.0 MKCl
13.2.0 M (NH4)2HPO4pH=8.2
14.3.5 M(NH4)2SO4
15.1.8 MLi2SO4
16.2.0 MMgCl2
17.2.5 MMgSO4
18.2.0 MKH2PO4/NaOHpH=5.5
19.2.0 MKNaTartrate
20.1.8 MNa3Citrate
21.5.0 MNaCl
22.4.0 MNaformate
23.100 %tert-butanol
24.100 %Ethanol
25.100 %Isopropanol

Solutions 1-22 also contain 1 mM Dithiothreitol, 1 mM NaN3,1 mM Ethylenediaminetetraacetic acid sodium salt (Na-EDTA).


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