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X-tal protocols

stock solutions X-tal fact2

Protein Detergent Methods Euipment Screen Drops Reference
1.H20
2.40 % (W/V)Polyethylene glycol (PEG) 6000
3.40 % (W/V) MME-PEG 5000
4.50 % (V/V)Jeffamine/HClpH=7.5
5.50 % (V/V)Polypropylene glycol P-425
6.50 % (V/V)2-Methyl-2,4-pentanediol
7.100 %Isopropanol
8.100 %Ethyleneglycol
9.50 % (V/V)Glycerol
10.0.9 MCitric acidpH=4.0
0.9 MBisTrisPropane
10 mMDTT,NaN3,Na-EDTA
11.0.9 MNa3CitratepH=9.0
0.9 MBisTrisPropane
10 mMDTT,NaN3,Na-EDTA
Adjust pH with solution 10.
12.3.5 M(NH4)2SO4
13.6.0 MCsCl
14.2.0 MCoSO4
15.4.0 MKCl
16.4.0 MLiCl2
17.5.0 MNaCl
18.2.0 MMgCl2
19.50 % (V/V)1,4-Dioxane
20.50 % (V/V)Dimethyl sulfoxide (DMSO)
21.100 %1-octanol
22.4.0 M1,6 Hexane-diol
23.10 % (W/V) ß-Octyl glucoside
24.0.1 % (W/V)NP-40

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